Journal: International Journal of Molecular Sciences

Article Title: Schizophyllum commune Exopolysaccharides Reduce Salmonella Gut Epithelial Invasion and Activate Macrophages Towards M1-Polarization

doi: 10.3390/ijms27104476

Figure Lengend Snippet: Percentage of host cell viability in different S. commune EPs concentration by an MTT assay. High doses (1000 and 2000 µg/mL) of S. commune CMU-01 EPs decreased human colonocyte (T84) cell viability ( A ) but not for murine macrophage (RAW264.7) ( C ). Commercially available schizophyllan (Biosynth ® ) EPs did not decrease the viability of both T84 and RAW264.7 cells ( B , D ). Bars represent the geometric mean with geometric standard deviation of at least eight independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Human colonic epithelium T84 (ATCC; CCL-248) and murine macrophage RAW264.7 (ATCC; TIB-71) cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Concentration Assay, MTT Assay, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Schizophyllum commune Exopolysaccharides Reduce Salmonella Gut Epithelial Invasion and Activate Macrophages Towards M1-Polarization

doi: 10.3390/ijms27104476

Figure Lengend Snippet: Dose-dependent effect of S. commune CMU-01 EPs in the reduction in Salmonella invasion to human colonocyte (T84) and activation of phagocytic activity of murine macrophage (RAW264.7). T84 and RAW264.7 cells were pretreated with three concentrations of S. commune EPS for 24 h prior to being infected with S. Typhimurium (MOI = 1) ( A , B ). Then, the gentamicin protection assay was used to determine the intracellular Salmonella counts (CFU/mL). Bars represent the geometric mean, with geometric standard deviation. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, a non-statistically significant difference.

Article Snippet: Human colonic epithelium T84 (ATCC; CCL-248) and murine macrophage RAW264.7 (ATCC; TIB-71) cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Activation Assay, Activity Assay, Infection, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Schizophyllum commune Exopolysaccharides Reduce Salmonella Gut Epithelial Invasion and Activate Macrophages Towards M1-Polarization

doi: 10.3390/ijms27104476

Figure Lengend Snippet: Immunomodulatory effect of S. commune CMU-01 EPs on human gut epithelium with and without S. Typhimurium infection. Human gut epithelium (T84) cells were pretreated with different concentrations of S. commune CMU-01 EPs for 24 h before Salmonella infection (MOI = 1) (shaded area) or phosphate-buffered saline (PBS) (non-shaded area). Fold changes in cytokine mRNA expressions ( IL8 , IL1b , IL10 , MIP3A , TNFa , and DECTIN1 ) were determined by quantitative PCR ( A – F ). Bars represent the geometric mean with geometric standard deviation of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, a non-statistically significant difference.

Article Snippet: Human colonic epithelium T84 (ATCC; CCL-248) and murine macrophage RAW264.7 (ATCC; TIB-71) cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Infection, Saline, Real-time Polymerase Chain Reaction, Standard Deviation

Journal: bioRxiv

Article Title: Lysosomal Alkalinization Selects for Metabolically Plastic, Motile Cancer Cells under Nutrient Stress

doi: 10.64898/2026.04.16.718649

Figure Lengend Snippet: A. Time-course viability curves of Caco-2, T84, and RKO cells were modeled using both logistic (solid lines) and exponential (dashed lines) fits at 24, 48, 72, and 96 hours. Panels represent (top) NR+Baf, (middle) ND+Baf, and (bottom) ND conditions. B. Phalloidin staining showing a remarkable increase in elongation in the ND+Baf Caco-2 cells (***p<0.001, *p<0.05, ANOVA). C . Phalloidin staining in RKO cells showing a significant increase in the elongation of ND cells as well as ND+Baf cells (****p<0.0001, **p<0.01, ANOVA). D . Phalloidin staining in T84 cells showing no change in the elongation of ND cells and an increase in the ND+Baf cells (**p<0.01, ANOVA). n=25 for elongation analyses. Scale bar: 50µm.

Article Snippet: Caco-2 cells were purchased from ŞAP Enstitüsü (Ankara, Turkey), RKO and T84 cells were purchased from ATCC.

Techniques: Staining